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. 2002 Oct;161(4):1135–1141. doi: 10.1016/s0002-9440(10)64390-8

Figure 3.

Figure 3.

Sequential extraction of τ proteins from AGD, AD, and PiD cases: Western blot analysis. Temporal cortex (AD, cases 8 and 9; PiD, cases 6 and 7; AGD, cases 1, 2, and 5) and entorhinal cortex and amygdala (AGD, case 4) with abundant τ pathology were studied after repeated extraction with buffers of increasing stringency as described in Materials and Methods. Lane 1: Buffer A (first extraction is shown); lane 2: 1% Triton X-100 in buffer A; lane 3: RIPA buffer; lane 4: 2% SDS; lane 5: 70% formic acid. A mixture of mAbs Tau14 and Tau46 were used for the Western blot analysis. An equal amount of material from each extraction step was loaded. Highly insoluble aggregated τ species were detected in AGD cases 2, 4, and 5 (arrowheads).