Immunolocalization of mononuclear and polymorphonuclear inflammatory cells during the course of choroidal neovascular membrane development after laser injury of the choroid. Mac-1 immunostaining (red) on frozen sections counterstained with bis-benzimide (blue) is absent in the choroid at day 3 (A, B), and appears in the impact at day 5 (C, D). Neutrophils (red) are present at day 3 (E, F), but not at day 5 (data not shown). White arrows localize the laser impact. Original magnifications: A, C, ×100; B, D, E, ×400; F, ×630.