Figure 8.

Six Different Mutants (gfs2, gfs3, gfs4, gfs5, gfs9, and gfs10) Other Than vsr1/gfs1 Alleles Were Isolated.

(A) Seeds of six gfs mutants (gfs2, gfs3, gfs4, gfs5, gfs9, and gfs10) were inspected with a confocal laser scanning microscope. GFP fluorescence was detected out of the cells of these mutant seeds, showing missorting of GFP-CT24. The GFP fluorescence image was not merged with the autofluorescent image (red) of PSVs. Each image of green and red fluorescence is shown in Supplemental Figure 1 online. Bars = 10 μm.

(B) Seeds of wild-type, gfs2, gfs3, gfs4, gfs5, gfs6, gfs9, and gfs10 plants were subjected to immunoblot analysis with anti-12S globulin antibody to determine the behavior of endogenous seed storage proteins in these seeds. These gfs mutants abnormally accumulated 12S globulin in precursors in seeds. p12S, 12S globulin precursors; 12S-α, α-subunit of mature 12S globulin. Molecular masses are given at left.

(C) Mutations of gfs1, gfs2, gfs3, gfs4, gfs5, gfs9, and gfs10 were mapped to different loci from each other: gfs1 to At3g52850 (VSR1), gfs2 to At2g26890 (KAM2/GRV2), and gfs10 to At4g35870.