Figure 4.

CLSM of Brassica Tapetum Cells of Developmental Stages 3 or 5 to Test for Colocalization of Flavonoids and Organelle-Specific Proteins.

Anthers were treated with DPBA for flavonoid fluorescence (shown in green) and then antibodies against calreticulin (markers of ER), oleosin (tapetosomes), F3′H (a flavonoid-synthesizing enzyme presumed to be located on the ER), V-PPase (vacuoles), or PAP (elaioplasts) for immunodetection (shown in red). Yellow/orange color in the merged images indicates colocalization.