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. 2007 Feb;19(2):433–444. doi: 10.1105/tpc.106.049221

Figure 5.

Figure 5.

Expression and Localization Studies of HUB1 and HUB2.

(A) RT-PCR analysis of the HUB1 and HUB2 transcripts in organs of 6-week-old Arabidopsis plants. R, roots; S, stem, ML, mature leaves; SL, senescent leaves; YL, young leaves; CL, cauline leaves; FB, flowers and buds; YS, young siliques. The ACTIN2 gene was used as a loading control.

(B) Expression analysis by an HUB1 promoter:GUS construct in transgenic Arabidopsis plants reveals GUS signals throughout the entire plant. The left panel shows an 8-d-old plant; the right top panel shows an inflorescence; the right middle panel shows part of a silique 12 d after pollination; and the right bottom panel shows an isolated embryo 12 d after pollination.

(C) HUB1 and HUB2 proteins are located in the nucleus. The top panels show YFP signals in confocal microscopic images of cells from transgenic Arabidopsis plants stably transformed with the P35S:YFP:HUB1 construct. The inset (magnification with a higher contrast) shows the position of the nucleus. The bottom panels show CFP and YFP signals in confocal microscopic images of N. benthamiana cells transiently expressing P35S:CFP:H2B (to detect the nucleus) and P35S:YFP:HUB2.