Figure 2.

RNA Gel Blot Analysis of AOX Gene Expression in Wild-Type and CMSII Plants Treated with Respiratory Inhibitors.

Plantlets were incubated in the complex IV inhibitor KCN (10 mM in water), the complex III inhibitor AA (100 μM dissolved in 1% DMSO), and the AOX inhibitor SHAM (10 mM in 1% DMSO). Water was used as control for KCN, and DMSO was used for the AA and SHAM treatments. RNA gel blot analysis was performed as described in Figure 1.

(A) Results of the full set of inhibitor treatments compared with water and DMSO controls. Experiments were repeated at least three times with similar results, and a typical experiment is shown.

(B) Less-exposed autoradiographs of RNA gel blots after KCN and AA treatments. These displays are from separate experiments compared with (A).