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. 2007 Feb;19(2):688–705. doi: 10.1105/tpc.106.048710

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Copyright © 2007, American Society of Plant Biologists

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Figure 9. — SOBER1 Enzyme Activity Is Required for Suppression of AvrBsT Resistance in Pi-0.

(A) In planta bacterial growth in Pi-0 T1 transgenic lines constitutively expressing Flag-SOBER1, Flag-SOBER1-H192A, Flag-SOBER1-S106A, or Flag-GUS. Leaves infected with a 10⁵ cfu/mL suspension of Pst DC3000 pVSP61(avrRpt2_1-100-avrBsT_11-350-HA) were sampled from individual plants at 0 d (black bars) and 3 d (gray bars) after inoculation (dpi). Flag-GUS Pi-0 T1 lines and untransformed Col-0 plants served as resistant and susceptible controls, respectively. Data for two independent T1 lines are presented. Similar results were obtained with four independent transformants per test construct.

(B) Immunoblot analysis of the uninfected, independent Pi-0 T1 transgenic plants used for (A). Total protein (∼50 μg) was analyzed by gel blot analysis using Flag antisera. Only SOBER1 protein expression is shown. Flag-GUS protein expression was detected in the appropriate lines (data not shown). Data presented were obtained from a series of immunoblots from a single day. The top bands represent nonspecific proteins, and the bottom band represents the Flag-tagged SOBER1 protein.