Figure 12.

TPI Transcript Accumulation in Systemic Leaves Doesn't Require Activating SIPK in Systemic Leaves.

Leaves at node +1 from rosette-stage N. attenuata were wounded with a pattern wheel; 20 μL of M. sexta OS (OS) was applied to the wounds. Treated leaves (+1, local) and systemic untreated leaves (−1) were harvested at indicated times. Four replicate leaves were pooled after harvesting.

(A) Kinase activity assay in both local and systemic leaves after elicitation.

(B) Transcript accumulation analyses of WIPK and TPI in local and systemic leaves by RNA gel blotting.