Figure 2.

SIPK and WIPK Regulate Transcript Accumulation of WRKY Genes.

N. attenuata plants were inoculated with Agrobacterium carrying pTV00 EV or constructs harboring a fragment of SIPK or WIPK to generate EV, SIPK-VIGS, and WIPK-VIGS plants, respectively. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS and regurgitants (W+OS) was applied to the wounds and harvested at the indicated times. Mean levels (±se) from five replicate plants of WRKY6 (A), WRKY7 (B), and SubD48 (C) transcripts in EV, SIPK-VIGS, and WIPK-VIGS plants after W+W and W+OS treatments were measured with q-PCR. Asterisks represent significantly different transcript levels between EV and SIPK-VIGS or WIPK-VIGS plants after the indicated times (n = 5, two-way ANOVA, Fisher's PLSD; *, P < 0.05; **, P < 0.01; ***, P < 0.001).