Figure 3.

SIPK and WIPK Regulate Transcript Accumulation of MAPKs.

N. attenuata plants were inoculated with Agrobacterium carrying pTV00 EV or constructs harboring a fragment of SIPK or WIPK to generate EV, SIPK-VIGS, and WIPK-VIGS plants, respectively. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS (W+OS) was applied to the wounds. Individual leaves from five replicate plants were harvested at the indicated times. Asterisks represent significantly different transcript levels between EV and VIGS plants at the indicated times ([A] and [B], unpaired t test; [C] to [E], two-way ANOVA, Fisher's PLSD; *, P < 0.05; **, P < 0.01; ***, P < 0.001).

(A) Mean WIPK transcript levels (±se) in EV and SIPK-VIGS plants after W+W and W+OS treatments as measured with q-PCR.

(B) Mean transcript levels (±se) of SIPK in EV and WIPK-VIGS plants after W+W and W+OS treatments as measured with q-PCR.

(C) to (E) Mean transcript levels (±se) of MPK4, Naf3, and Naf6 in EV, SIPK-VIGS, and WIPK-VIGS plants after W+W and W+OS treatments, respectively, as measured with q-PCR.