Figure 4.

SIPK and WIPK Mediate Transcript Accumulation of CDPKs.

N. attenuata plants were inoculated with Agrobacterium carrying pTV00 EV or constructs harboring a fragment of SIPK or WIPK to generate EV, SIPK-VIGS, and WIPK-VIGS plants, respectively. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS (W+OS) was applied to the wounds. Individual leaves from five replicate plants were harvested at the indicated times. Mean (±se) transcript levels in five replicate plants of CDPK2 (A), CDPK4 (B), CDPK5 (C), and CDPK8 (D) in EV, SIPK-VIGS, and WIPK-VIGS plants after W+W and W+OS treatments were measured with q-PCR. Asterisks represent significantly different transcript levels between EV and SIPK-VIGS or WIPK-VIGS plants after the indicated times (two-way ANOVA, Fisher's PLSD; *, P < 0.05; **, P < 0.01; ***, P < 0.001).