Figure 5.

SIPK and WIPK Mediate Levels of JA and Transcript Accumulation of JA Biosynthetic Genes.

N. attenuata plants were inoculated with Agrobacterium carrying pTV00 EV or constructs harboring a fragment of SIPK or WIPK to generate EV, SIPK-VIGS, and WIPK-VIGS plants, respectively. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS (W+OS) was applied to the wounds. Individual leaves from five replicate plants were harvested at the indicated times. Asterisks represent significantly different levels between EV and SIPK-VIGS or WIPK-VIGS plants after the indicated times (two-way ANOVA, Fisher's PLSD; *, P < 0.05; **, P < 0.01; ***, P < 0.001).

(A) Mean (±se) JA concentrations were measured using HPLC–tandem mass spectrometry (MS/MS). FW, fresh weight.

(B) and (C) Mean transcript levels (±se) of LOX3 and AOS as measured with q-PCR.