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. 2007 Mar;19(3):1096–1122. doi: 10.1105/tpc.106.049353

Figure 9.

Figure 9.

SIPK and WIPK Mediate Levels of TPI Activity and Transcript Levels of PAL.

N. attenuata plants were inoculated with Agrobacterium carrying pTV00 EV or constructs harboring a fragment of SIPK or WIPK to generate EV, SIPK-VIGS, and WIPK-VIGS plants, respectively. Asterisks represent significant differences between EV and SIPK-VIGS or WIPK-VIGS plants after the specific treatments (n = 5, two-way ANOVA, Fisher's PLSD; *, P < 0.05; **, P < 0.01; ***, P < 0.001).

(A) Mean (+se) TPI activity in EV, SIPK-VIGS, and WIPK-VIGS plants. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS (W+OS) was applied to the wounds, and leaves from five replicate plants were harvested individually 3 d after treatments. Untreated plants served as controls.

(B) Mean transcript levels (±se) of PAL in EV, SIPK-VIGS, and WIPK-VIGS plants after W+W and W+OS treatments as measured with q-PCR. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS (W+OS) was applied to the wounds.