SIPK and WIPK Mediate Levels of TPI Activity and Transcript Levels of PAL.
N. attenuata plants were inoculated with Agrobacterium carrying pTV00 EV or constructs harboring a fragment of SIPK or WIPK to generate EV, SIPK-VIGS, and WIPK-VIGS plants, respectively. Asterisks represent significant differences between EV and SIPK-VIGS or WIPK-VIGS plants after the specific treatments (n = 5, two-way ANOVA, Fisher's PLSD; *, P < 0.05; **, P < 0.01; ***, P < 0.001).
(A) Mean (+se) TPI activity in EV, SIPK-VIGS, and WIPK-VIGS plants. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS (W+OS) was applied to the wounds, and leaves from five replicate plants were harvested individually 3 d after treatments. Untreated plants served as controls.
(B) Mean transcript levels (±se) of PAL in EV, SIPK-VIGS, and WIPK-VIGS plants after W+W and W+OS treatments as measured with q-PCR. Leaves were wounded with a pattern wheel; 20 μL of either water (W+W) or M. sexta OS (W+OS) was applied to the wounds.