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Effects of N-Terminal Deletion and Mutation of Ser-82 and Ser-97 Residues in the St RBOHB N Terminus on Kinase Activities.
Soluble proteins were prepared from potato tubers inoculated with P. infestans (1.0 × 105 zoospores/mL). In-gel kinase assay was performed using the N-terminal region of St RBOHB with introduced N-terminal deletion (A) or site-directed mutagenesis of Ser-82 and/or Ser-97 as substrate (B).
