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. 2007 Mar;19(3):1007–1022. doi: 10.1105/tpc.107.050633

Figure 4.

Figure 4.

Phenotypes of Plants Overexpressing Chlase Versions under Low-Intensity Light.

(A) Squash plants were infected with empty viral vector (AGII), viral vector harboring the full-length Chlase gene (AGII-Chlase), or viral vector harboring the N-terminally deleted form of the Chlase gene (AGII-ChlaseΔN). Plants were grown for 10 d after inoculation under low-intensity fluorescent light (15 μE), and leaf 3 of each plant was documented.

(B) Kinetics of in vitro Chlase activity was measured from crude extracts of leaf 4 of the plants used for (A), as described in Methods. Data points represent AGII-Chlase (squares), AGII-ChlaseΔN (triangles), and AGII (circles).

(C) Squash plants were infected with viral vector harboring the N-terminally deleted form of the Chlase gene in the wild-type form (AGII-ChlaseΔN) or mutated at Ser-147 to encode Thr (AGII-ChlaseΔN S147T). Plants were grown for 10 d after inoculation under low-intensity fluorescent light (15 μE), and leaf 3 of each plant was documented.