Figure 1.

Scheme of the GUS Constructs in the Binary Vector pOCA30.

(A) The T-DNA region of the binary vector pOCA30 contains the CaMV 35S promoter with duplicated enhancers. GUS constructs were inserted behind the 35S promoter. LB, left border; RB, right border; E35S promoter, CaMV 35S promoter with duplicated enhancers; GUS, β-glucuronidase; 35S terminator, the CaMV 35S transcription terminator; nos terminator, the transcription terminator from the nos gene of A. tumefaciens; EcoRI/ClaI fragment, the ∼0.8-kb EcoRI-ClaI DNA fragment from pOCA30 located 3′ to the insertion site of the GUS constructs.

(B) DNA sequences of the CaMV 35S terminator and the Agrobacterium nos gene terminator used in the GUS gene constructs.