Figure 4.

Analysis of Total and Full-Length GUS Transcripts in T1 pGt_s and pGGGt_s Transformants in the sde1-1 Mutant Background.

Total RNA was isolated from four randomly selected T1 transformants with a single-copy T-DNA insertion for each construct in the sde1-1 background and probed first with the GUS gene fragment (A). After stripping the GUS probe, the blot was rehybridized with the CaMV 35S terminator sequence (B). GUS activities for individual transformants analyzed are shown at the bottom of the blot. The ethidium bromide stain of rRNA is shown for each lane to allow assessment of equal loading.