Figure 2.

Coomassie Blue–Stained Protein Gel of the Recombinant Protein Expression and Purification.

Lane 1, total extract from bacterial cells without MAP18 expression (25 μg); lane 2, total extract from bacterial cells for the protein expression without isopropylthio-β-galactoside (IPTG) induction (25 μg); lane 3, total extract from IPTG-induced bacterial cells for the protein expression (25 μg); lane 4, purified GST recombinant protein in eluate (25 μg); lane 5, purified recombinant protein after the removal of GST (4 μg).