Figure 1.

Antibodies Used in This Study.

(A) Positions of peptides that were used for immunization. For each protein, the top line represents the predicted protein encoded by the full-length Arabidopsis (At) or tomato (Sl) cDNA, whereas the bottom line(s) represents the positions of the peptides of the tomato protein that were used for immunization.

(B) Characterization of the antibodies. Immunoblot strips carrying molecular weight markers (M), proteins from tomato anthers containing PMCs in early meiotic prophase (Early), pachytene (Pach), or late meiotic prophase (Late), or proteins from tomato root tips (Root) were incubated in Ponceau S (P) or in affinity-purified antibodies from sera elicited against peptides shown in (A): lane 1, anti-Sl SMC1 (serum 5); left strip, preimmune serum; right strip, anti-SMC1 antibodies; lane 2, anti-Sl SMC3 (serum 623), anti-SMC3 antibodies; lane 3, anti-Sl MLH1 C-terminal peptide (serum 8); left strip: preimmune serum; right strip, anti-MLH1 antibodies; lane 4, anti-Sl CENPC (serum 20); left strip, preimmune serum; right strip, anti-CENPC antibodies. The arrow indicates the top of the gel, and the numbers to the left represent the molecular masses in kilodaltons of the marker proteins.