Figure 7.

Identification of Genes Responding to 9-HOT and Analyses of Gene Expression.

(A) Cluster analysis of genes changing their expression in roots treated with 9-HOT. Cluster 1 contains upregulated genes. Clusters 2A, 2B, and 2C are highly, slightly, and moderately downregulated genes, respectively. A complete list is provided in Supplemental Table 1 online.

(B) List of genes selected from microarray data for further characterization. Shown are locus designations, descriptions of putative encoded proteins, and differential expression ratios between roots grown in the absence of 9-HOT and roots grown for 3 or 5 d in 9-HOT–containing medium.

(C) RT-PCR analysis of genes in control and 9-HOT–treated roots, leaves subjected to 9-HOT treatment, and leaves responding to inoculation with the avirulent bacterium Pst DC3000 avrRpm1 or the virulent strain Pst DC3000. Gene At1g43170 encoding RPL3A was used to normalize transcript levels in each sample.