Figure 5.

Accumulation of RGL2 Protein during a Time Course of Seed Imbibition.

RGL2 protein was detected by protein gel blot analysis.

(A) Ler seeds were incubated for 6 d at 4°C and then time points were at indicated intervals after transfer to 22°C (blot exposure >30 min). The correlation between RGL2 protein accumulation and radicle emergence (%) was determined. RGL2 protein disappeared within 12 h, before radicle emergence.

(B) Time-course analysis of RGL2 protein in wild-type and mutant backgrounds. Protein gel blot analysis (exposure time 5 to 10 min) of protein isolated from seeds imbibed at 4°C for 1, 3, 5, and 6 d and from seeds first imbibed for 3 d at 4°C then transferred to 22°C for indicated number of days. Protein from dry seeds was also examined. Seed samples used were Ler (28 months old), ga1-3 (25 months old), sly1-10 (27 months old, #3), after-ripened (A) sly1-2 (26 months old, #21), dormant (D) sly1-2 (0.5 months old, #35), Ws (0.5 months old), and sly1-t2 (0.5 months old, #12). Asterisks indicate nonspecific bands.