Figure 6.

RGL2 mRNA Accumulation during a Time Course of Seed Imbibition for Wild-Type, ga1-3, and sly1 Mutant Seeds.

RT-PCR analysis of RGL2 mRNA expression in seeds imbibing using the same time courses in Figure 6 of stratified (4°C for 1, 3, 5, and 6 d) seeds and seeds imbibing at 22°C after 3 d of stratification at 4°C. Seed samples used were identical to Figure 6 and include Ler (28 months old), ga1-3 (25 months old), sly1-10 (27 months old, #3), after-ripened (A) sly1-2 (26 months old, #21), dormant (D) sly1-2 (0.5 months old, #35), Ws (0.5 months old), and sly1-t2 (0.5 months old, #12).