Figure 4.

Protein Interactions between AP3 and PI Homologs of A. vulgaris as Determined by Growth on Selective SD Medium and Quantification of Secreted α-Galactosidase Activity (see Supplemental Methods Online).

Aquilegia and Arabidopsis proteins were cloned into both binding domain (BD) and activation domain (AD) constructs. For each protein pair listed, AD/BD indicates that protein 1 was fused with AD and protein 2 was fused with BD. BD/AD indicates the opposite situation. The colonies shown in the –HLT (−HisLeuTrp) columns represent dilution series (10⁵, 10⁴, and 10³ colony-forming units) of each strain grown on –HLT SD medium supplemented with 20 mM 3-amino-1,2,4-triazole. n/a, not applicable. For the graph, the dark gray bars show the results from analyses of α-galactosidase activity where the first protein listed was in the AD construct and the second protein was in the BD construct. The light gray bars are for the reverse BD/AD combination. Error bars represent sd across three independent replicates.