Figure 7.

Locus-Specific RT-PCR on RNA Prepared from Organs of VIGS-Treated Flowers.

(A) RNA samples from five tissue types were tested: flowers that were treated with TRV2-AqvPI-AqvANS but did not show silencing (NS), silenced flowers that were treated with TRV2-AqvANS (ANS−), first whorl organs from strongly silenced flowers treated with TRV2-AqvPI-AqvANS (w1), second whorl organs from strongly silenced flowers treated with TRV2-AqvPI-AqvANS (w2), and carpeloid organs from strongly silenced flowers treated with TRV2-AqvPI-AqvANS (car).

(B) Individual RT-PCR for 6 unsilenced and 12 silenced tissue samples. The sample numbers correspond to the bud number and whorl from which the sample was taken. For instance, sample 2.2 was a second whorl organ from the second flower to be characterized. We analyzed 33 individual flowers showing some degree of silencing as well as 12 flowers showing no silencing from the same plants. In some cases, silenced and unsilenced organs from the same whorl were analyzed separately (e.g., 3.1a and 3.1b). Unsilenced samples included sepals (whorl 1), petals (whorl 2), and stamens (whorl 3), while silenced samples included sepals (whorl 1), petaloid or sepaloid organs in whorl 2, and carpeloid organs in internal whorls (c). Normalized expression values are shown for each sample.