Figure 1. Generation of Lck^creIL-4Rα^−/lox Mice.

(A) Mouse breeding strategy. IL-4Rα^lox/lox BALB/c mice were intercrossed with transgenic BALB/c mice expressing Cre-recombinase under control of the Lck promoter and IL-4Rα^−/− BALB/c mice to generate Lck^creIL-4Rα^−/lox mice. The “loxed” IL-4Rα allele, gray arrows; deleted allele, black arrows.

(B) Genotyping of Lck^creIL-4Rα^−/lox mice. The deleted IL-4Rα PCR yields a product of 471 bp, LoxP, 188 bp (loxed), and 94 bp (WT), and Cre-specific a 450-bp product.

(C) Phenotypic analysis. WT (solid line), IL-4Rα^−/− (gray line), and Lck^creIL-4Rα^−/lox BALB/c mice (dashed line) LN cells were stained for expression of IL-4Rα. T cell subsets were identified using anti-CD3, anti-CD4/CD8, or δ-TCR. B cells, anti-CD19. DCs, CD11^c/I-A^d. Macrophages, F4/80/I-A^d.

(D) Efficiency of IL-4Rα deletion. The ratio of IL-4Rα exon 5 and exon 8 alleles was determined by real-time PCR from genomic DNA purified from CD4⁺ or CD19⁺ cells. PCR products of amplified genomic DNA from real-time PCR reactions (75 cycles) were visualized on agarose gel. Data is representative of 2 independent experiments with triplicate values ± SD.