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Direct sequencing of 234-bp (A and B) and 129-bp (C) PCR products from GIST 2. Note the hardly visible (A) and artificially elevated (B) “tail sequence” of mutant KIT allele. Elevated peaks of mutant KIT allele could not be easily distinguished from the elevated background. C: A shift of sequence detected in 129-bp PCR assay indicating duplication is emphasized by an arrow. WT and mutant KIT allele codons demarcating beginning of duplication are indicated by codon numbers.
