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Direct sequencing of 234-bp (A) and 129-bp (B) PCR products from GIST 3. A: Note the lack of mutant KIT allele sequence in the products from 234-bp PCR assay. B: A shift of sequence detected in 129-bp PCR assay indicating duplication is emphasized by an arrow. WT and mutant KIT allele codons demarcating beginning of duplication are indicated by codon numbers.
