Table 1.
PCR Assays Used to Evaluate KIT Exon 11
| Primer | Nucleotide position* | Primer sequence | PCR | Temperature (°C) | Size (bp) |
|---|---|---|---|---|---|
| CK10.7F | g.75575_75595 | 5′-CCATTTATTTGTTCTCTCTCC-3′ | CK10.7F/CK11.3R | 50 | 234 |
| CK11.1F | g.75670_75690 | 5′-CAGTGGAAGGTTGTTGAGGAG-3′ | CK11.1F/CK11.3R | 55 | 129 |
| CK11.3R | g.75789_75808 | 5′-AGCCCCTGTTTCATACTGAC-3′ | |||
| CK11.3.5R | g.75825_75845 | 5′-AGTCACTGTTATGTGTACCCA-3′ | CK10.7F/CK11.3.5R | 50 | 271 |
F, forward; R, reverse.
*
Nucleotide position based on human KIT (locus HSU63834) sequence available in GenBank at http:www.ncbi.nlm.nih.gov.