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. 2007 Apr;9(2):272–276. doi: 10.2353/jmoldx.2007.060133

Figure 1.

Figure 1

Schematic diagram of the ARMS-PCR assay. Two forward and two reverse primers are used in different combinations to generate three potential PCR products. Primers FO and RO flank the exon 14 of the JAK2 gene, resulting in a band of 463 bp to control for DNA quality and quantity. Primers Fwt and RO amplify a wild-type allele, generating a band of 229 bp, and primers FO and Rmt generate a band of 279 bp from the mutant allele. Primer sequences are shown below the diagram. The intended mismatches in Fwt and Rmt are shown in lowercase, and genotype-specific nucleotides are underlined.