Table 1.
Comparison of PCR Conditions of the Original and Modified ARMS PCR Assays
| Condition types | Original | Modified |
|---|---|---|
| Reaction conditions | ||
| dNTP | 200 μmol/L | 120 μmol/L |
| Primer FO | 1.0 μmol/L | 0.4 μmol/L |
| Primer RO | 1.0 μmol/L | 0.3 μmol/L |
| Primer Fwt | 0.5 μmol/L | 0.5 μmol/L |
| Primer Rmt | 0.5 μmol/L | 1.0 μmol/L |
| DNA polymerase | AmpliTaq Gold, 1 U | HotStart, 1 U |
| Template DNA | 25 ng | 100 ng |
| Total volume | 25 μl | 25 μl |
| Cycling conditions | ||
| Denaturing | 94°C, 1 minute | 94°C, 30 seconds |
| Annealing | 60°C, 1 minute | 58°C, 45 seconds |
| Extension | 72°C, 1 minute | 72°C, 45 seconds |
| Cycle number | 30 | 40 |