Table 2.

Validation of the Modified ARMS-PCR Assay by Pyrosequencing

Specimens	Modified ARMS	Pyrosequencing (% of mutant T allele*)
1	Normal	0
2	Normal	0
3	Normal	4.3
4	Mutant only	95
5	Normal	2.6
6	Normal	2.0
7	Normal	2.1
8	Mutant + normal	23.7
9	Mutant + normal	34.3
10	Mutant + normal	39.8
11	Mutant + normal	58.5
12	Normal	1.0
13	Normal	0.9
14	Mutant + normal	70.1
15	Mutant + normal	27.7
16	Normal	1.6
17	Normal	1.0
18	Mutant + normal	77.6

^*Less than 5% of T by pyrosequencing is scored as normal.