Table 2.
Nucleotide Sequences of Primers Used for Multiplex PCR
| Gene | Primer
|
|
|---|---|---|
| Designation | Sequence | |
| Round 1 | ||
| Vγ1-8 | Vγ1-8F1ex | 5′-TCTTCCAACTTGGAAGGGAGA-3′ |
| Vγ9 | Vγ9F1ex | 5′-TCTGCAACATCTGTATATTGGTATC-3′ |
| Vγ10-11 | Vγ10-11F1ex | 5′-CTGGTACCGGCAGAAACCAAA-3′ |
| Jγ1/Jγ2 | Jγ1/Jγ2R1ex | 5′-TAAACATTATTACATTATTCCAGTT-3′ |
| Jγp1/Jγp2 | Jγp1/Jγp2R1ex | 5′-TCTATCAGTTTTTCATTACTGGAAT-3′ |
| Jγp | JγpR1ex | 5′-CTCCCATCCCTTCTTTACATTGCA-3′ |
| Round 2 | ||
| Vγ1-8 | Vγ1-8F2in | Cy5-5′-GAAGGCCCCACAGCGTCTTC-3′ |
| Vγ9 | Vγ9F2in | Cy5-5′-AAGGAATCTGGCATTCCGTCAG-3′19 |
| Vγ10 | Vγ10F2in | Cy5-5′-TGTCTCAACAAAATCCGCAGCT-3′ |
| Vγ11 | Vγ11F2in | Cy5-5′-GGAAGACTAAGAAACTTGAGGT-3′ |
| Jγ1/Jγ2 | Jγ1/Jγ2R2in | 5′-TCTTCCGATACTTACCTGTGACAAC-3′ |
| Jγp1/Jγp2 | Jγp1/Jγp2R2in | 5′-GAAGTTACTATGAGCCTAGTCCCTT-3′19 |
| Jγp | JγpR2in | 5′-AAGCTTTGTTCCGGGACCAAATAC-3′19 |
Cy5, corresponding to fluorescently labeled primer. Bold font emphasizes that there is a modification with Cy5.