Table 1.

Peptides Identified at 5% Error Rate in Proteins Enriched by Immunoprecipitation and Immunoaffinity Chromatography

Chromato- graphy	Name	Description	IPI No.	Peptide	X corr	Charge state
IA	O52B6	Olfactory receptor 52 B6	303388	LHEPMY*IFLSMLASADVLLSTTTMPKAL	1.38	1
IA	IgG	Immunoglobulin heavy chain variable region	7893	TAIY*Y*CARISGDRGAFDMWGQG	2.58	2
IA	FSH-R	Follicle-stimulating hormone receptor precursor	328365	RARSTY*NLKKLPT	2.62	2
IA	CEP2	Centrosomal protein 2	160622	LQKEVVLLQAQLTLERKQKQDY*I	2.53	2
IA	E2G1	Ubiquitin-conjugating enzyme E2G 1	514050	KDY*PLRPPKMKFITEIWHPNVDK	2.54	2
IA	CLCN5	Chloride channel 5	294056	FNTSKGGELPDRPAGVGVY*SA	2.52	2
IA	DNAH8	Dynein	216653	FEPSFCFY*TGYKIPLCKTLDQY*FEYI	1.23	1
IA	Statip1	STAT3-interacting protein 1	15560	VLHPSQRYVVAVGLECGKICLY*TWKKTD	2.12	1
IA	RASGRF1	Ras protein-specific guanine nucleotide-releasing factor 1	16784	PIITGGKALDLAALSCNSNGY*TSMY*SAMS	2.05	1
IA	FPR1	Formylpeptide receptor 1	328644	LPTNTSGGTPAVSAGY*LFL	1.48	1
IP	NCOR2	Nuclear receptor co- repressor 2	1735	ACY*EESLKSRPGTASSSGGSIAR	3	2
IP	IgG1	Immunoglobulin heavy chain variable region	7906	IIPFSGIAY*YAQK	2.59	2
IP	DOCK7	Dedicator of cytokinesis protein 7	513791	LLDLLYLCVSCFEY*KGK	2.55	2
IP	CLK2	CDC-like kinase 2	478691	SSY*DDRSSDRRVY*D	1.45	1
IP	IGHV4–61	Immunoglobulin-μ heavy chain variable region	4995455	WIRQPAGKGLEWIGRIY*TTGSTNSNPS	1.6	1
IP	EIF2C3	Eukaryotic translation initiation factor 2C 3	304181	Y*TLQLKY*PHLPCLQVGQEQK	1.38	1
IP	ARTS-1	Type 1 tumor necrosis factor receptor shedding aminopeptidase	165949	VEWIKFNVGMNGYY*	2.62	2
IP	MYBPC3	Myosin binding protein C gene	292412	AELIVQEKKLEVY*QSIA	2.56	2
IP	HLA-Cw	Major histocompatibility complex class I antigen	144014	LEAARAAEQLRAY*LEGTCVEWLR	2.58	2
IP	CCR1	Chemokine (C-C motif) receptor 1	27685	PLY*SLVFVIGLVGNILVVLV	1.38	1
IP	CDC2L5	Cell division cycle 2-like 5 isoform 2	456970	LPRSPSPY*SR	2.19	1

Peptides identified by LC-MS/MS in immunoprecipitation (IP)- and immunoaffinity (IA)-enriched samples were analyzed for corresponding proteins using INTERACT and Protein Prophet and compared with the International Protein Index (IPI) database. Listed are select IP- and IA-enriched tyrosine phosphoproteins including protein name, description, National Center for Biotechnology Information accession no., and identified peptide sequence. Y*, phosphotyrosine.