Figure 3.

Detection of mutant BRAF alleles in plasma samples from patients with metastatic melanoma. Representative electropherogram results from 10 patients with metastatic melanoma. MS-PCR results are shown in the top panel; control amplification of the entire BRAF exon 15 is shown in the bottom panel. In this group, 7 of 10 (70%) patients had detectable BRAF mutations in their plasma samples. The placental DNA samples were used as controls and were negative for the mutant BRAF and positive for BRAF exon 15, as expected. The y axes are relative fluorescence units.