Skip to main content
. 2004 May;6(2):101–107. doi: 10.1016/S1525-1578(10)60497-7

Figure 2.

Figure 2

Wild-type and mutant K-ras sequences detected in human urine and disease tissue by restriction-enriched polymerase chain reactions (RE-PCR). A: The predicted pattern of digested products. B: The results of analysis of DNA derived from the urine of an individual with no diagnosis of cancer (Zu) and from tumor tissue (AAt), non-tumor tissue that was 1 cm apart from the tumor (AAadj), serum (AAs), and urine (AAu) from an individual with CRC. C: The sensitivity and specificity of the assay by analysis of the reconstruction standards (1.5, 15, and 150 copies of SW480 genome per 50 ng of HepG2 DNA), and 50 ng HepG2 DNA and 5 ng SW480 alone, as indicated. D: Detection of mutated K-ras DNA in CRC tissue. CRC disease tissue sections from five CRC patients (Ot, AAt, AMt, BQt, and DGt) were subjected to DNA isolation and the RE-PCR assay for mutated K-ras DNA. MW: DNA MW markers. This represents the data from three independent experiments.