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. 2004 May;6(2):108–114. doi: 10.1016/S1525-1578(10)60498-9

Figure 2.

Figure 2

Determination of C. albicans load in blood culture fluids inoculated with a low colony count. Culture bottles inoculated with 3.67 CFU of exponential-phase C. albicans were placed in the BACTEC system for increasing times or until an alarm signal indicated positivity. At each interval of 1 hour, an aliquot was withdrawn, DNA extracted and submitted to PCR amplification in the presence of 0.5% BSA. The mean value of the CFU values of two independent experiments was calculated. Each point in each experiment was tested in duplicate. The detection time indicating positivity was determined to be 31 hours by the BACTEC system.