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Temperature gradient effect on peak resolution (in 100 seconds scale). A: Wild-type control (exon 4, 270 bp); B: R117H heterozygous (exon 4) with 50 to 55°C gradient; C: R117H heterozygous (exon 4) with 55 to 60°C gradient; D: wild-type control (exon 11, 175 bp); E: G551D heterozygous (exon 11) with 50 to 55°C gradient; F: G551D heterozygous (exon 11) with 55 to 60°C gradient.
