Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of dilution with PCR buffers (1× buffer containing 1.5 mmol/L Mg+2; 10× buffer containing 15 mmol/L Mg+2) on peak resolution using amplicon G551D heterozygous (exon 11) as an example. A: Dilution (1 in 2) using 1× PCR buffer; B: 1 in 4 dilution using 1× PCR buffer; C: 1 in 1.25 dilution using 10× PCR buffer; D: 1 in 2 dilution using 10× PCR buffer.
