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. 2005 May;7(2):226–235. doi: 10.1016/S1525-1578(10)60549-1

Figure 2.

Figure 2

hMLH1 and hMSH2 group probes. A: LG1, LG2, and LG3 represent the hMLH1 group 1, group 2, and group 3 probes, respectively. Open blocks represent the exons in the group probe. BamHI and NotI restriction enzymes located at both ends of the inserts were used to release the inserts. B: SG1, SG2, and SG3 are the hMSH2 group 1, group 2, and group 3 probes, respectively. Instead of the pcR2.1-TOPO vector, SG1 was subcloned into the pGEM-T easy vector and the SG1 probe was released by digestion with the NotI restriction enzyme.