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. 2005 May;7(2):226–235. doi: 10.1016/S1525-1578(10)60549-1

Figure 3.

Figure 3

hMLH1 Southern blot hybridization. A: Southern blot analysis of patients 1 to 3 (lanes 1 to 3) with the LG1, LG2, and LG3 probes. All samples were digested by three restriction enzymes (EcoRI, BglII, and HindIII). Each band is labeled with the exon used in that probe. It is important to note that each fragment may include multiple exons. See Table 1 for overlap of exons within the same fragment. Several polymorphisms can be observed (eg, EcoRI for e13, EcoRI for e12). B: Densitometric analysis of the LG1 HindIII digest of patients 1 to 3 (lanes 1 to 3). Patient 2, peak 1 demonstrates a 50% reduction in density, corresponding to a deletion in exon 13. C: Densitometric analysis of the LG3 HindIII digest of patients 1 to 3. A junction fragment with the exon 12 probe is apparent in patient 2.