Table 1.
hMLH1 and hMSH2 Exon Migration Sizes
| Exon |
hMLH1 migration size (kb)
|
hMSH2 migration size (kb)
|
||||
|---|---|---|---|---|---|---|
| EcoRI | BglII | HindIII | EcoRI | BglII | HindIII | |
| 1 | 11 | 8 | 4.6 | 8.8 | 5.5 | 10 |
| 2 | 11 | 8 | 5.6 | 2.2 | 2.1 | 10 |
| 3 | 4.5 | ND | 6.8 | 2.2 | 2.1 | 2.7 |
| 4 | 4.5 | 3 | 6.8 | 8.8 | 10 | 0.5 |
| 5 | 10 | 15 | 6.8 | 8.8 | 10 | 1.7 |
| 6 | 10 | 15 | 4.5 + 1.4 | 8.8 | 10 | 6.6 |
| 7 | 10 | 15 | 4.5 | 9 | 14 + 0.6 | 1.6 |
| 8 | 10 | 15 | 4.5 | 6.2 | 3.7 | 7/5.3* |
| 9 | 10 | 15 | 1.1 + 0.8 | 3.2 + 2.6 | 5 + 0.7 | 4.4 |
| 10 | 3.8 | 15 | 2.8 | 4.3 | 5 | 1.1 |
| 11 | 2.8 | 15 | 6.2 | 2.6 | 7 | 7.0 |
| 12 | 11/8.2* | 9 | 6.2 + 7.4 | 1.9 | 7 | 1.4 |
| 13 | 11/8.2* | 9 | 7.4 | 1.9 | 3.9 | 10 |
| 14 | 3.6 | ND | 3.6 | 1.2 | 3.9 | 10 |
| 15 | 5 | 15 | 2.3 | 2.8 | 6.5 | 10 |
| 16 | 7 | 15 | 3.3 | 5.3 | 6.5 | 10 |
| 17 | 7 | 15 | 3.3 | |||
| 18 | 7 | 15 | 3.3 | |||
| 19 | 7 | 15 | ND | |||
*
The number following the/is the size (kb) of an alternative band resulting from a polymorphic restriction site within this fragment. ND means that fragment was too small to detect. If a restriction site is within an exon, 2 fragments may be detected. In such cases, the two fragment sizes are indicated (eg. 4.5 + 1.4).