Figure 2.

Linearity of the synthetic SSX RNA amplified by competitive NASBA. Four different amounts of SSX WT RNA were analyzed to evaluate the linearity and the dynamic range. The copy number of diluted WT RNA was calculated by extrapolating signal ratio to the calibration curve (data points and bars indicate mean ± SD; n = 5). The assay system showed the linearity in the range between 10² and 10⁷ copies. All within-run CVs in this assay were under 5%, except the CV for 10² copies of RNA (7.93%).