Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
KRAS Pyrosequencing assay design. A: PCR amplifies a segment of KRAS exon 1 containing codons 12 and 13. The reverse PCR primer is biotinylated. B: KRAS-PF1 Pyrosequencing primer can detect common codon 12 mutations. C: KRAS-PF2 Pyrosequencing primer can detect the rest of the codon 12 mutations. D: KRAS-PF3 Pyrosequencing primer can detect codon 13 mutations. WT, wild type; Mut, mutant.
