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. 2005 Aug;7(3):413–421. doi: 10.1016/S1525-1578(10)60571-5

Figure 6.

Figure 6

Comparison of Pyrosequencing and BigDye Terminator sequencing. A: Cell line DNA mixing study. A1: The c.35G>T (codon 12 GTT) mutation can be observed by both methods. A2: The c.35G>T (codon 12 GTT) mutation in the 2% mix can easily be observed by Pyrosequencing (left) but is subtle in BigDye sequencing (right). A3 and A4: The c.38G>A (codon 13 GAC) mutation can barely be observed by Pyrosequencing (left) but cannot be detected by BigDye sequencing (right). B: Paraffin colon tumor DNA mixing study. B1 and B2: The c.35G>T (codon 12 GTT) mutation can be barely observed by Pyrosequencing (left) but cannot be detected by BigDye sequencing (right). B3 and B4: The c.35G>A (codon 12 GAT) mutation can barely be observed by Pyrosequencing (left) but cannot be detected by BigDye sequencing (right). B5 and B6: The c.34G>A (codon 12 AGT) mutation can readily be observed by Pyrosequencing (left) but cannot be detected by BigDye sequencing (right).