Table 2.
Results of Paraffin Colon Cancer Tissue DNA Mixing Study
| Fraction of cancer DNA | Diagnosis | Dideoxy (BigDye) sequencing | Pyrosequencing |
|---|---|---|---|
| 50% | Correct | 15/15 (100%) | 15/15 (100%) |
| Wild type (incorrect) | 0/15 (0%) | 0/15 (0%) | |
| Indeterminate | 0/15 (0%) | 0/15 (0%) | |
| 30% | Correct | 14/15 (93%) | 15/15 (100%) |
| Wild type (incorrect) | 0/15 (0%) | 0/15 (0%) | |
| Indeterminate | 1/15 (7%) | 0/15 (0%) | |
| 20% | Correct | 8/15 (53%)* | 15/15 (100%)* |
| Wild type (incorrect) | 5/15 (33%) | 0/15 (0%) | |
| Indeterminate | 2/15 (13%) | 0/15 (0%) | |
| 10% | Correct | 6/15 (40%)† | 15/15 (100%)† |
| Wild type (incorrect) | 6/15 (40%) | 0/15 (0%) | |
| Indeterminate | 3/15 (20%) | 0/15 (0%) | |
| 5% | Correct | 7/15 (47%) | 10/15 (67%) |
| Wild type (incorrect) | 6/15 (40%) | 5/15 (33%) | |
| Indeterminate | 2/15 (3%) | 0/15 (0%) |
The numbers in the first, second, and third row for each mixture represent the fractions of individual runs with correct diagnosis, with wild-type diagnosis, and with indeterminate diagnosis, respectively.
*
Statistical significance, P = 0.006.
†
Statistical significance, P = 0.0007.