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Fluorescence measurements derived using the SmartCycler for 10-fold serial dilutions of GBS (5 to 50,000 CFU/ml) spiked into neonatal whole-blood samples and analyzed by 16S rDNA PCR. Site ID numbers represent samples tested in duplicate at the following concentrations: A1 to 2, 5 × 104 CFU/ml; A3 to 4, 5 × 103 CFU/ml; A5 to 6, 5 × 102 CFU/ml; A7 to 8, 50 CFU/ml; A9 to 10, 5 CFU/ml; and A11 to 12, negative controls lacking target DNA.
