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. 2005 Nov;7(5):613–622. doi: 10.1016/S1525-1578(10)60595-8

Figure 2.

Figure 2

The heteroplasmic mtDNA deletions determined by Southern analysis and real-time qPCR. A:Southern blot analysis. The number on the top is patient’s number. The number at the bottom is the percentage of deletion molecules. The percentage of deletion heteroplasmy is the intensity of mtDNA with deletion (bottom band) divided by the sum of the intensity of mtDNA with deletion and the intact mtDNA (top band). The intensity of the DNA bands is measured using Scion Image for Windows (β 4.02) software (http://www.scioncorp.com). B: The correlation between the percentages of deletion mutant measured by real-time qPCR and Southern blot. The calculation of percent of mtDNA deletion by real-time qPCR was described in the footnote of Table 2. Only the data points from the deleted region(s) were included in the plot: ATP8 (9 filled circles) and ND4 (12 open circles). Patient 21 in Table 2, which has multiple deletions, was not included in the plot. The linear regression analysis was performed using SigmaStat for Windows Version 2.03, and the graph was plotted using SigmaPlot 2000 for Windows Version 6.00. The P value is <0.001.