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. 2005 Nov;7(5):613–622. doi: 10.1016/S1525-1578(10)60595-8

Figure 3.

Figure 3

Detection of mtDNA multiple deletions by Southern analysis (A) and PCR (B). Lanes 1 to 6 are normal control and patients 101 to 105, respectively. Lane 7 in B is no template control in PCR. A: Southern blot analysis. Total DNA was digested with HindIII followed by electrophoresis in 0.8% agarose gel and Southern analysis. The blot was hybridized with mtDNA probes (see Materials and Methods). B: PCR using primers mtF7234-R16133 (I), mtF8295-14499 (II), and mtF5681-R14686 (III). M, 100-bp DNA marker.