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. 2005 Nov;7(5):613–622. doi: 10.1016/S1525-1578(10)60595-8

Figure 4.

Figure 4

Measurement of mtDNA content. A: Southern analysis. The upper blot was hybridized with mtDNA probe, and the lower blot was hybridized with 18S rDNA probe. The numbers on the top (1 to 18) are DNA samples from patients 201 to 218, respectively. The numbers at the bottom are ratios of signal intensities of mtDNA to 18S rDNA. Total muscle DNA (0.15 μg) was digested with EagI followed by Southern analysis. B through D: MtDNA content determined by real-time qPCR using β2M (B), AIB1(C), or β-actin gene (D) as nuclear gene reference and their correlation with the mtDNA content determined by Southern blot analysis using 18S rDNA as the reference nuclear gene. mtDNA is the mean of copy number of mtDNA from tRNA leuUUR and D-loop region determined by standard curves.