Table 2.
Comparison of Sequence and Fragment Analysis Methods to Estimate the FMR1 Gene Triplet Number
| Subjects | Repeat structure | No. of sequenced triplets | Length (bp) | No. of estimated triplets* |
|---|---|---|---|---|
| B960 | (CGG)12 | 12 | 244.66 | 8 |
| C383 | (CGG)10-AGG-(CGG)9 | 20 | 265.65 | 15 |
| A241 | (CGG)10-AGG-(CGG)9 | 20 | 264.58 | 15 |
| C009 | (CGG)10-AGG-(CGG)10 | 21 | 268.60 | 16 |
| B771 | (CGG)12-AGG-(CGG)10 | 23 | 275.70 | 18 |
| B513 | (CGG)9-AGG-(CGG)14 | 24 | 276.96 | 19 |
| C244 | (CGG)10-AGG-(CGG)16 | 27 | 285.00 | 21 |
| C229 | (CGG)9-AGG-(CGG)9-AGG-(CGG)9 | 29 | 290.26 | 28 |
| B185 | (CGG)9-AGG-(CGG)9-AGG-(CGG)9 | 29 | 290.43 | 23 |
| A341 | (CGG)10-AGG-(CGG)9-AGG-(CGG)9 | 30 | 294.28 | 24 |
| B258 | (CGG)9-AGG-(CGG)9-AGG-(CGG)12 | 32 | 298.23 | 26 |
| B514 | (CGG)9-AGG-(CGG)24 | 34 | 306.43 | 28 |
| C163 | (CGG)9-AGG-(CGG)9-AGG-(CGG)6-AGG-(CGG)10 | 37 | 314.76 | 31 |
| A965 | (CGG)10-AGG-(CGG)9-AGG-(CGG)9-AGG-(CGG)9 | 40 | 322.91 | 34 |
| C044 | (CGG)9-AGG-(CGG)9-AGG-(CGG)23 | 43 | 330.38 | 36 |
| B179 | (CGG)9-AGG-(CGG)9-AGG-(CGG)26 | 46 | 336.19 | 38 |
| C789 | (CGG)9-AGG-(CGG)9-AGG-(CGG)45 | 65 | 385.94 | 55 |
| Clones | ||||
| FX-C2 | (CGG)17 | 17 | 257.48 | 12 |
| FX-D5 | (CGG)18 | 18 | 260.50 | 13 |
| FX-D1 | (CGG)23 | 23 | 274.57 | 18 |
| FX-F3 | (CGG)24 | 24 | 279.73 | 19 |
| FX-D6 | (CGG)26 | 26 | 281.90 | 20 |
| FX-G9 | (CGG)28 | 28 | 284.87 | 21 |
| FX-F10 | (CGG)10-AGG-(CGG)9-AGG-(CGG)9 | 30 | 293.62 | 24 |
| FX-A12 | (CGG)10-AGG-(CGG)9-AGG-(CGG)9 | 30 | 293.65 | 24 |
| FX-G5 | (CGG)9-AGG-CAG-(CGG)25 | 36 | 309.29 | 29 |
| FX-G10 | (CGG)9-AGG-(CGG)14-AGG-(CGG)7-AGG-(CGG)14-AGG-(CGG)7 | 55 | 359.31 | 46 |
*
The number of triplets is estimated using the formula: No. of triplets = (bp − 221)/3.